The application of modern non-destructive testing methods on grain quality testing had important implications for grain production, distribution and sale. In the paper, five test methods of modern electronic information technology, which were near-infrared spectroscopy, electronic nose, machine vision, magnetic resonance and acoustic vibrations, were described, the research progress of near-infrared spectroscopy, electronic nose technology and machine vision technology in grain quality nondestructive testing was reviewed with focuses. The application of modern non-destructive testing methods on grain quality testing could reduce costs and improve detection accuracy, furthermore, make it combined with modern computer technology and data processing, which would play an important role in agriculture.