Quadratic rotation-orthogonal composite experimental design was applied in this experiment to obtain the ultraweak luminescence property of mint affected by multilateral growth environmental factors including illumination, temperature, humidity, and moisture. The influence models of four factors on luminescence were established. Analysis showed that different factors and their interactions had different effect on luminous value, among which the interaction of illumination and humidity had the most significant influence on luminescence of mint (p ≤ 0.01). And the maximum luminous value was obtained when ten stick of lamp tube (intensity of illumination value 4229 Lx) was used, mean temperature, humidity and water were 35 ℃, 85% and 90 mL/d respectively. On the base of that, linear effects and their interactions that affected ultraweak luminescence were also studied.