In order to realize the acquisition of high-resolution and high-precision image in agricultural field environment, the agricultural field environment high-quality image remote acquisition system was designed, based on Canon DSLR camera. This paper made detailed design about core algorithm, including remote acquisition system architecture, camera access, image transmission and command response. The scheme used Fit-PC to control Canon 550D DSLR camera for image acquisition and WIFI wireless transmission module to transmit collected images to the remote server. Meanwhile, the server could also remotely operate camera to collect images in different ways through wireless transmission command, according to different needs. The experimental results showed that the system had three main advantages as high resolution, precision and image transmission rate in collecting images. The resolution of images collected in each node was up to 5184×3456, and the effective breadth of Canon camera image was 17 times as the Logitech camera. In addition, at a distance of 16 meters, image captured by Logitech camera could not see the target while the same one captured by Canon camera could still clearly display. Moreover, the average speed of wireless images transmission to the server was 0.97MB/S. Therefore, this system could meet the requirements of high-quality image acquisition and transmission in agricultural field environment.